Solid-Phase Nested Deletion: A New Subcloning-less Method for Generating Nested Deletions
نویسندگان
چکیده
منابع مشابه
A new PCR based method for the generation of nested deletions.
We have developed a simple, PCR-based protocol, random primed/anchored-PCR (RPA-PCR), that allows the selective amplification and efficient cloning of segments that are adjacent to any known sequence. We demonstrate that RPA-PCR can be used to prepare a nested set of evenly spaced deletions suitable for DNA sequencing. However, it should also be possible to use this technique for a number of ot...
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ژورنال
عنوان ژورنال: DNA Research
سال: 1995
ISSN: 1340-2838
DOI: 10.1093/dnares/2.4.175